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KMID : 0369819940240030131
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Jorunal of Korean Pharmaceutical Sciences
1994 Volume.24 No. 3 p.131 ~ p.137
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Delivery System of Daunorubicin by Red Blood Cells
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함성호/Ham SH
송경/고건일/김재백/손동환/Song K/Ko GI/Kim JB/Sohn DH
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Abstract
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Drug delivery system by the use of red blood cells was established to sustain the release of drugs in the circulatory system by the intravenous injection. The entrapment method by the preswelling technique was re-examined and evaluated for searching the new entrapping conditions without hemolysis. The addition of 4 volume of 0.6×hank′s balanced salt solution (HBSS) into 1 volume of 50% red blood cells suspension did not induce the hemolysis and change the hematocrit level in this experimental condition (within 15 min). Most of daunorubicin could be entrapped into red blood cells within 15 min. While the intracellular adenosine triphosphate (ATP) level followed by the entrapment was reduced to 86% of normal ATP level, the membrane fluidity and the shape factor of red blood cells were not altered. The release rate of daunorubicin from red blood cells was affected by the hemolysis under this condition. To maintain the intracellular ATP in red blood cells, the new reaction buffer was made With the addition of ATP and sodium pyruvate during the entrapment procedure because the hemolysis during the release test would reflect the loss of intracellular ATP that might result in the decrease of the viability in vivo. The addition of ATP raised the intracellular ATP level, which protect the hemolysis during the release test.
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KEYWORD
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Daunorubicin, Red blood cell, Hemolysis, Membrane fluidity
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